fadR [2019-09-02 11:32:40]

transcriptional repressor (TetR family), regulation of fatty acid degradation

locus: BSU_28550

pI: 6.12

mw: 21.83 kDa

protein length: 194 aa

gene length: 585 bp

function: regulation of fatty acid degradation

product: transcriptional repressor

essential: no

synonyms: ysiA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 2,917,957 2,918,541

The protein

Protein family

• [SW|TetR family]

[SW|Domains]

• HTH tetR-type domain (aa 5-65) (according to UniProt)

Effectors of protein activity

• long chain fatty acids act as inducers that release FadR from its operator sites [Pubmed|24356978]

Structure

• [PDB|3WHB] [pubmed|24356978]

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000195 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]-[gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]-[gene|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|fadB]-[gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]-[gene|ACA8FF923D72CC99F447E47F6A1381FEAD9DAB09|etfA]
  description: [Pubmed|17189250]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21398533], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|21398533], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by long chain acyl-CoA (C14 ... C20) ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]

• genes: [gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]-[gene|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|fadB]-[gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]-[gene|ACA8FF923D72CC99F447E47F6A1381FEAD9DAB09|etfA]
  description: [Pubmed|17189250]

  regulation

  • induced by long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]

Biological materials

Mutant

• MGNA-B008 (ysiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1007 NBRP B. subtilis, Japan]
• 1A853 ( ''fadR''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A853&Search=1A853 BGSC]
• BKE28550 ([gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTTCATCGTTCCTTCC, downstream forward: _UP4_AATAAGTAAAGGAGTTCTGT
• BKK28550 ([gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTTCATCGTTCCTTCC, downstream forward: _UP4_AATAAGTAAAGGAGTTCTGT

References

Reviews

• 17919287,24006471

Original publications

• 17189250,21398533,24356978